Application

Research CategoryApoptosis & Cancer

This Anti-TSPAN8 Antibody, clone CO-029 (1116NS-29) is validated for use in Immunohistochemistry (Paraffin), Flow Cytometry, Western Blotting, Affinity Binding Assay for the detection of TSPAN8.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected TSPAN8 in Caco-2 human epithelial colorectal adenocarcinoma cells.
Affinity Binding Assay: A representative lot detected surface CO-029 antigen (TSPAN8) expression among a panel of human tumor cell lines by radioimmune assay (RIA) and mixed-hemadsorption assay (MHA) (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837).
Flow Cytometry Analysis: A representative lot detected surface CO-029 antigen (TSPAN8) immunoreactivity predominantly among tumor cells in pancreatic adenocarcinoma tissues-derived cell cultures (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852).
Immunocytochemistry Analysis: A representative lot detected CO-029 antigen (TSPAN8) immunoreactivity colocalized with those of integrins alpha6 and beta4 by dual fluorescent immunocytochemistry staining of 8.18 human pancreatic cancer cells (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852).
Immunocytochemistry Analysis: A representative lot detected CO-029 antigen (TSPAN8) immunoreactivity colocalized with that of integrin beta4 by dual fluorescent immunocytochemistry staining of human pancreatic cancer Capan1 cells, as well as human colon cancer HT29 and WIDR cells (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852).
Immunohistochemistry Analysis: A representative lot detected an upregulated CO-029 antigen (TSPAN8) immunoreactivity in frozen pancreatic adenocarcinoma tissue sections than normal pancreatic tissue sections (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852).
Western Blotting Analysis: A representative lot detected CO-029 antigen (TSPAN8) in integrin alpha3 immunoprecipitates from 8.18 human pancreatic cancer cell lysates (Gesierich, S., et al. (2005). Clin. Cancer Res. 11(8):2840-2852)
Western Blotting Analysis: A representative lot detected the endogenous CO-029 antigen (TSPAN8) in SW948 human colon cancer cell lysate as well as the exogenously expressed CO-029 antigen in transfected COS cells (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837).

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tetraspanin-8 (UniProt P19075; also known as Transmembrane 4 superfamily member 3, Tspan-8, Tumor-associated antigen CO-029) is encoded by the TSPAN8 (also known as TM4SF3) gene (Gene ID 7103) in human. CO-029 is a member of the transmembrane 4 or tetraspanin family of cell-surface 4-transmembrane glycoproteins involved in the regulation of cell activation, growth, and motility. Tetraspanin-8 (CO-029 antigen) was originally characterized as a tumor-associated antigen expressed in gastric, colorectal, and pancreatic cancer cells and exert tumor progression-promoting activity. In metastatic pancreatic and colorectal carcinoma cell lines, PKC activation enhances the colocalization of Tetraspanin-8 and tetraspanin CD151 with integrin α6β4 and subsequent internalization of this integrin-tetraspanin complex, resultinig in decreased cell-matrix adhesion and increased cell migration. Tetraspanin-8 knockdown in HT29 colon cancer cells is reported to significantly reduce cell migratory ability as a result of an upregulation of both integrin-dependent cell-matrix adhesion and calcium-dependent cell-cell adhesion. Human tetraspanin-8 is a four-transmembrane (10-33, 58-72, 84-109, 206-230) protein with both its N- and C-terminal ends (a.a. 1-9 and 231-237) exposed at the cytoplasmic side.

Immunogen

SW1116 human colorectal carcinoma cells.

Epitope: Extracellular domain.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G purified.

Format: Purified

Quality

Evaluated by Immunohistochemistry in human colon cancer tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected TSPAN8 in human colon cancer tissue section.

Specificity

Clone CO-029 (originally 1116NS-29) detected the glycosylated target bands in CO-029 antigen (TSPAN8) cDNA-transfected, but not untransfected COS cells (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

26.04 kDa calculated. ~27-34 kDa reported (Szala, S., et al. (1990). Proc. Natl. Acad. Sci. U. S. A. 87(17):6833-6837) due to glycosylation.